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Chemical Engineering

Ronald J. Neufeld, Ph.D.

Professor Emeritus

Tel: (613) 533-2827
Email: neufeld@queensu.ca
Office: Bioscience Complex Room 1443

Professor Emeritus

Main

Please note that as of January 1, 2016, Dr. Neufeld is no longer accepting new graduate students, post-doctoral fellows or research associates.  

Research Interests

The micro- and nanoencapsulation of biologically active materials enables stabilization, retention, immunoprotection, controlled or targeted delivery, and cell growth in a protected environment. Cells, tissues and bioactives that we have experience with include mammalian cells, microorganisms, enzymes, nucleotides, genomic DNA, therapeutic peptides and growth factors, and molecular-targeting therapeutics directed toward growth factor receptors. As tissues and biologicals are labile, encapsulation matrix/membrane materials and methods often involve use of natural polymers such as polysaccharides and proteins, thus avoiding the use of solvents, pH extremes and highly reactive polymerization conditions. The control of polymer matrix/membrane properties achieve specific therapeutic goals, such as bioactive retention and protection in the gastrointestinal tract, mucoadhesion and translocation across the intestinal membrane, and thus oral delivery of a protein-based drug as an example.

This research is interdisciplinary and collaborative, thus graduate students and researchers have opportunity to conduct research in collaborating labs. Recent and active research areas are listed below, with names of collaborators.

Sustained delivery of molecular-targeting therapeutics in cancer treatment

Prof. Myron Szewczuk, Department of Biomedical and Molecular Sciences, Queen's University

Prof. Dérick Rousseau, Department of Chemistry and Biology, Ryerson University

Hybrid biomaterials for self-assembly and controlled release

Prof. Michael Cunningham, Department of Chemical Engineering, Queen's

Prof. Pascale Champagne, Department of Civil Engineering, Queen's

Prof. Ralph Whitney, Department of Chemistry, Queen's

Long term sustained delivery of growth factors for treatment of burn wounds

Prof. Manuela Martins-Green, Department of Cell Biology and Neuroscience, University of California, Riverside

Prof. Annelise Barron, Department of Bioengineering, Stanford University

Extracellular matrix-based micro and nanofibre microbead scaffold for cell growth and implantation

Dr. Jean-Pierre Hallé and Dr. Euridice Carmona, Maisonneuve-Rosemont Hospital, Laboratory for Bioartificial Therapies, Montreal

Prof. Annelise Barron, Department of Bioengineering, Stanford University

Oral delivery of nanoparticulate insulin in control of diabetes

Dr. Christiane Damgé, Université Louis Pasteur, Strasbourg, France

Prof. Catarina Pinto Reis, Laboratory of Nanoscience and Biomedical Nanotechnology, ULHT, Lisbon

Prof. Bruno Sarmento, Instituto Nacional de Engenharia Biomédica, Porto

Design fundamentals for encapsulation processes

Prof. Denis Poncelet, ONIRIS, Nantes, France

Publications

Reprint requests to: neufeld@queensu.ca

Refereed Publications (Reviews listed following refereed publications)

137.     Allison, N.S., A.J. Brissenden, M.R. Szewczuk and R.J. Neufeld. Combinatorial and sequential delivery of gemcitabine and oseltamivir phosphate from implantable poly(lactic-co-glycolic acid) cylinders disables human pancreatic cancer cell survival. Drug Design, Development and Therapy, in press, June 23, 2017.

 136.     Akasov, R., S. Haq, F. Haxho, V. Samuel, S.V. Burov, E. Markvicheva, R.J. Neufeld and M.R. Szewczuk. 2016. Sialylation transmogrifies human breast and pancreatic cancer cells into 3D multicellular tumor spheroids using cyclic RGD-peptide induced self-assembly. Oncotarget, 7: 66119-66134.

135.     Kapishon, V., S. Allison, R.A. Whitney, M.F. Cunningham, M. Szewczuk and R.J. Neufeld. 2016. Oseltamivir-conjugated polymeric micelles prepared by RAFT living radical polymerization as a new active tumor targeting drug delivery platform. Biomaterials Science, 4: 511-521.

134.     Dhall, S., J.P. Silva, Y. Liu, M. Hrynyk, M. Garcia, A. Chan, J. Lyubovitsky, R.J. Neufeld and M. Martins-Green. 2015. Release of insulin from PLGA-alginate dressing stimulates regenerative healing of burn wounds in rats. Clinical Science, 129: 1115-1129.

133.     Hrynyk, M., J.P. Ellis, F. Haxho, S. Allison, J.A.M. Steele, S. Abdulkhalek, R.J. Neufeld and M.R. Szewczuk. 2015. Therapeutic designed poly (lactic-co-glycolic acid) cylindrical oseltamivir phosphate-loaded implants impede tumor neovascularization, growth and metastasis in mouse model of human pancreatic carcinoma. Drug Design, Development and Therapy, 9: 4573-4586.

132.     Kapishon, V., R. Whitney, P. Champagne, M.F. Cunningham and R.J. Neufeld. 2015. Polymerization induced self-assembly of alginate based amphiphilic graft copolymers synthesized by single electron transfer living radical polymerization. Biomacromolecules, 16: 2040-2048. (ACS Editor’s Choice and amongst top 10 most read papers in journal over past 12 months).

131.     Perignon, C., G. Ongmayeb, R.J. Neufeld, Y. Frere and D. Poncelet. 2015. Microencapsulation by interfacial polymerization: Membrane formation and structure. J. Microencapsulation, 32: 1-15.

130.     Haxho, F., S. Allison, F. Alghamdi, L. Brodhagen, V. Kuta, S. Abdulkhalek, R.J. Neufeld and M.R. Szewczuk. 2014. Oseltamivir phosphate monotherapy ablates tumor neovascularization, growth and metastasis in mouse model of human triple-negative breast adenocarcinoma. Breast Cancer: Targets and Therapy, 6: 191-203.

129.     Abdulkhalek, S., O. Geen, L. Brodhagen, F. Haxho, F. Alghamdi, S. Allison, D. Simmons, R.J. Neufeld and M.R. Szewczuk. 2014. Transcriptional factor snail controls tumor neovascularization, growth and metastasis in mouse model of human ovarian carcinoma. Clinical and Translational Medicine, 3:28-43.

128. Aziz, S., J. Gill, P.R.L. Dutilleul, R.J. Neufeld and S. Kermasha. 2014. Microencapsulation of krill oil using complex coacervation. J. Microencapsul., 31: 774-784. (Most downloaded paper in journal for 2015)

127. O'Shea, L.K., S. Abdulkhalek, S. Allison, R.J. Neufeld and M.R. Szewczuk. 2014. Therapeutic targeting of Neu1 sialidase with oseltamivir phosphate (Tamiflu®) disables cancer cell survival in human pancreatic cancer with acquired chemoresistance. OncoTargets and Therapy, 7: 117-134.

126. Bowey, K., B. Swift, L.E. Flynn and R.J. Neufeld. 2013. Characterization of biologically active insulin-loaded alginate microparticles prepared by spray drying. Drug Development and Industrial Pharmacy, 39: 457-465.

125. Steele, J.A.M., A.E. Barron, E. Carmona, J-P. Hallé and R.J. Neufeld. 2012. Encapsulation of protein microfiber networks supporting pancreatic islets. Journal of Biomedical Materials Research: Part A,100: 3384-3391.

124. Hrynyk, M., M. Martins-Green, A.E. Barron and R.J. Neufeld. 2012. Alginate-PEG sponge architecture and role in the design of insulin release dressings. Biomacromolecules, 13: 1478-1485.

123. Woitiski, C.B., R.J. Neufeld, A.F. Soares, I.V. Figueiredo, F. Veiga and R.A. Carvalho. 2012. Evaluation of hepatic glucose metabolism via gluconeogenesis and glycogenolysis after oral administration of insulin nanoparticles. Drug Development and Industrial Pharmacy, 38: 1441-1450..

122. Pinto Reis, C., R.J. Neufeld, F. Veiga, I.V. Figueiredo, J. Jones, A.F. Soares, P. Nunes, C. Damgé and R.A. Carvalho.2012. Effects of an oral insulin nanoplex on hepatic glucose metabolism assessed by 13C and 2H isotopomer analysis. J. Microencapsulation, 29: 167-176.

121. Woitiski, C.B., B. Sarmento, R.A. Carvalho, R.J. Neufeld and F. Veiga. 2011. Facilitated nanoscale delivery of insulin across intestinal membrane models. International Journal of Pharmaceutics, 412: 123-131.

120. Erdinc, B. and R.J. Neufeld. 2011. Protein micro and nanoencapsulation within glycol chitosan/Ca2+/alginate matrix by spray drying. Drug Development and Industrial Pharmacy, 37(6): 619-627.

119. Woitiski, C.B., R.J. Neufeld, F. Veiga, R.A. Carvalho and I.V. Figueiredo. 2010. Pharmacological effect of orally delivered insulin facilitated by multilayered stable nanoparticles. European Journal of Pharmaceutical Sciences, 41: 556-563.

118. Chan, A and R.J. Neufeld. 2010. Tuneable semi-synthetic alginate for absorptive encapsulation and controlled release of protein therapeutics. Biomaterials, 31: 9040-9047.

117. Hrynyk, M., M. Martins-Green, A.E. Barron and R.J. Neufeld. 2010. Sustained and prolonged topical delivery of bioactive human insulin for potential treatment of cutaneous wounds. International Journal of Pharmaceutics, 398: 146-154.

116. Chan, A. and R.J. Neufeld. 2009. Modeling the controllable pH-responsive swelling and pore size of networked alginate based biomaterials. Biomaterials, 30: 6119-6129.

115. Woitiski, C.B., F. Veiga, A.J. Ribeiro and R.J. Neufeld. 2009. Design for optimization of nanoparticles integrating biomaterials for orally dosed insulin. European Journal of Pharmaceutics and Biopharmaceutics, 73: 25-33.

114. Woitiski, C.B., R.J. Neufeld, A.J. Ribeiro and F. Veiga. 2009. Colloidal carrier integrating biomaterials for oral insulin delivery: Influence of component formulation on physicochemical and biological parameters. Acta Biomaterialia, 5: 2475-2484.

113. Chan, A.W., R.A. Whitney and R.J. Neufeld. 2009. Semisynthesis of a controlled stimuli-responsive alginate hydrogel. Biomacromolecules, 10: 609-616.

112. Reis, C.P., I.V. Figueiredo, R.A Carvalho, J. Jones, P. Nunes, A.F. Soares, C.F. Silva, A.J. Ribeiro, F.J. Veiga, C. Damgé, A.M.S. Cabrita and R.J. Neufeld. 2008. Toxicological assessment of orally delivered nanoparticulate insulin. Nanotoxicology, 2: 206-217.

111. Chan, A.W., R.A. Whitney and R.J. Neufeld. 2008. Kinetic controlled synthesis of pH-responsive network alginate. Biomacromolecules, 9: 2536-2545.

110. Woitiski, C.B., R.A. Carvalho, A.J. Ribeiro, R.J. Neufeld and F. Veiga. 2008. Strategies toward improved oral delivery of insulin nanoparticles via gastrointestinal uptake and translocation. BioDrugs, 22: 223-237.

109. Pinto Reis, C., F.J. Veiga, A.J. Ribeiro, R.J. Neufeld and C. Damgé. 2008. Nanoparticulate biopolymers deliver insulin orally eliciting pharmacological response. J. Pharm. Sci., 97: 5290-5305.

108. Yi, Y., S. Kermasha and R.J. Neufeld. 2008. Nanoporous sol-gel supports enzymatic hydrolysis of chlorophyll in organic media. In: Biomolecular Catalysis: Nanoscale Science and Technology. Kim, J., S.H. Kim, and P. Wang (Eds.), ACS Symp. Series 986, invited paper, 199-213.

107. Pinto Reis, C., A.J. Ribeiro, F. Veiga, R.J. Neufeld and C. Damgé. 2008. Polyelectrolyte biomaterial interactions provide nanoparticulate carrier for oral insulin delivery. Drug Delivery, 15: 127-139.

106. Strodthoff, P.A., S. Karboune, R.J. Neufeld and S. Kermasha. 2007. Optimization of chlorophyllase-catalyzed hydrolysis of chlorophyll in monophasic organic solvent media. Appl. Biochem. Biotechnol. 142: 263-275.

105. Sarmento, B., A. Ribeiro, F. Veiga, D. Ferreira and R.J. Neufeld. 2007. Oral insulin bioavailability contained in polysaccharide nanoparticles. Biomacromol. 8: 3054-3060.

104. Sarmento, B., A. Ribeiro, F. Veiga, P. Sampaio, R.J. Neufeld and D. Ferreira. 2007. Alginate-chitosan nanoparticles are effective for oral insulin delivery. Pharm. Res. 24: 2198-2206.

103. Yi, Y., R.J. Neufeld and S. Kermasha. 2007. Controlling sol-gel properties enhancing entrapped membrane protein activity through doping additives. J. Sol-Gel Sci. Technol. 43: 161-170.

102. Pinto Reis, C., A.J. Ribeiro, S. Houng, F. Veiga and R.J. Neufeld. 2007. Nanoparticulate delivery system for insulin: design, characterization and in vitro/in vivo bioactivity. Eur. J. Pharmaceut. Sci., 30: 392-397. (Ranked 5 of 25 most downloaded articles from journal on ScienceDirect, for period April-June, 2007.)

101. Sarmento, B., A.J. Ribeiro, F. Veiga, D.C. Ferreira and R.J. Neufeld. 2007. Insulin-loaded nanoparticles are prepared by alginate ionotropic pre-gelation followed by chitosan polyelectrolyte complexation, J. Nanosci. Nanotechnol., 7: 1-9.

100. Gu, F., R.J. Neufeld and B. Amsden. 2007. Sustained release of bioactive therapeutic proteins from a biodegradable elastomeric device. J. Cont. Rel., 117: 80-89.

99. Orgaz, B., R.J. Neufeld and C. SanJose. 2007. Single-step biofilm removal with delayed release encapsulated Pronase mixed with soluble enzymes. Enz. Microbial Technol., 40: 1045-1051.

98. Gu, F., R.J. Neufeld and B. Amsden. 2007. Maintenance of vascular endothelial growth factor and potentially other therapeutic proteins bioactivity during a photo-initiated free radical cross-linking reaction forming biodegradable elastomers. Eur. J. Pharm. Biopharm., 66: 21-27.

97. Pinto Reis, C., A.J. Ribeiro, R.J. Neufeld and F. Veiga. 2007. Alginate microparticles as a novel carrier for oral insulin delivery. Biotechnol. Bioeng., 96: 977-989.

96. Pinto Reis, C., R.J. Neufeld, S. Vilela, A.J. Ribeiro and F. Veiga. 2006. Review and current status of emulsion/dispersion technology using an internal gelation process for the design of alginate particles. J. Microencapsul., 23: 245-257 (Listed as 1 of 5 most read papers in Journal to August 2011).

95. Yi, Y., S. Kermasha and R.J. Neufeld. 2006. Characterization of sol-gel entrapped chlorophyllase. Biotechnol. Bioeng., 95: 840-849.

94. Pinto Reis, C., R.J. Neufeld, A.J. Ribeiro and F. Veiga. 2006. Nanoencapsulation II. Biomedical applications and current status of peptide and protein nanoparticulate delivery systems. Nanomedicine: Nanotechnology, Biology and Medicine, 2: 53-65.(Ranked 9th of 25 most downloaded articles from journal, April - June, 2006 on Science Direct. Ranked as 1st of 25 most downloaded articles, July - September, 2006. Ranked as 5 of 25 from October - December, 2006. Ranked as 4 of 25 from January to March, 2007. Ranked as 8 of 25 from April to June, 2007. Ranked as 10 of 25 from July to September, 2007. Ranked as 13 of 25 from October to December, 2007. Ranked 18 of 25 from January to March 2008. Ranked 20/25 from April to June 2008 Ranked 24/25 from April to June 2009.)

93. Pinto Reis, C., Ronald J. Neufeld, A.J. Ribeiro and F. Veiga. 2006. Nanoencapsulation I. Methods for preparation of drug-loaded polymeric nanoparticles. Nanomedicine: Nanotechnology, Biology and Medicine, 2: 8-21 (Ranked 1st of 25 most downloaded articles from journal, April - June, 2006 on Science Direct. Ranked as 2nd of 25 most downloaded articles, July - September, 2006. Ranked as 4 of 25 from October - December, 2006. Ranked as 1 of 25 from January - March, 2007. Ranked as 4 of 25 from April - June, 2007. Ranked as 7 of 25 from July to September, 2007. Ranked as 8 of 25 from October to December, 2007. Ranked 5 of 25 from January to March, 2008. Ranked 5/25 from April to June 2008. Ranked 9/25 from October to December 2008. Ranked 5/25 from January to March 2009. Ranked 4/25 from April to June 2009. Ranked 4/25 from July to September 2009. Ranked 5/25 from October to December 2009. Ranked 5/25 from January to March 2010. Ranked 7/25 from April to September 2010. Ranked 10/25 from October to December, 2010. Ranked 8/25 from January to March 2011. Ranked 11/25 from April to June 2011. Ranked 7/25 from October to December 2012. Ranked 7/25 for all 2012. Ranked 6/25 for January to March, 2013. Ranked 6/25 April to June 2013. Ranked 5/25 July to September 2013, Ranked 4/25 October to December 2013, Ranked 6/25 from January to December 2013. Ranked 4/25 from January to March 2014. Ranked 3/25 from April to June 2014 Ranked 3/25 from July to September, 2014. Ranked 3/25 from October to December 2014. Ranked 3/25 for 2014. Ranked 4/25 most downloaded papers in area of General and Internal Medicine in Elsevier Journals, for January to December 2014. Ranked 4/25 from January to March 2015. Ranked 2/25 from April to June 2015. Ranked 5/25 from July to September 2015. Cited 258 times to Nov. 30, 2015).

92. Reis, C.P., R.J. Neufeld, A.J. Ribeiro and F. Veiga. 2006. Design of insulin-loaded alginate nanoparticles: Influence of the calcium ion on polymer gel matrix properties. Chem. Ind. Chem. Eng. Quarterly, 12: 47-52.

91. Sarmento, B., S. Martins, A. Ribeiro, F. Veiga, R.J. Neufeld and D. Ferreira. 2006. Development and comparison of different nanoparticulate polyelectrolyte complexes as insulin carriers. Intern. J. Peptide Research and Therapeutics, 12: 131-138.

90. Gu, F., R.J. Neufeld and B. Amsden. 2006. Osmotic driven release kinetics of bioactive therapeutic proteins from a biodegradable elastomer are linear, constant, similar and adjustable. Pharm. Res., 23: 782-789.

89. Chan, A.W.J., I. Mazeaud, T. Becker and R.J. Neufeld. 2006. Granulation of subtilisin by internal gelation of alginate microspheres for application in detergent formulation. Enz. Microbial. Technol. 38: 265-272.

88. Yi, Yunyu, Ronald J. Neufeld and S. Kermasha. 2006. Optimization of tetramethoxysilane-derived sol gel entrapment protocol stabilizes active chlorophyllase. J. Sol-Gel Sci. Techn., 38: 251-259.

87. Karboune, S., R.J. Neufeld and S. Kermasha. 2005. Immobilization and biocatalysis of chlorophyllase in selected organic solvent systems. J. Biotechnol. 120, 273-283.

86. Yi, Y., S. Kermasha and R.J. Neufeld. 2005. Matrix physicochemical properties affect activity of entrapped chlorophyllase. J. Chem. Technol. Biotechnol., 80: 1395-1402.

85. Liu, Z.M., T. Becker and R.J. Neufeld. 2005. Spherical alginate granules formulated with quick-release active subtilisin. Biotechnol. Prog., 21: 568-574.

84. Gu, F., H.M. Younes, A.O.S. El-Kadi, R.J. Neufeld and B.G. Amsden. 2005. Sustained interferon-γ delivery from a photocrosslinked biodegradable elastomer, J. Cont. Rel., 102: 607-617.

83. Chan, A.W.J., T. Becker and R.J. Neufeld. 2005. Subtilisin absorptive encapsulation and granulation. Process Biochemistry, 40: 1903-1010.

82. Moslemy, P., D. Millete, S. Guiot and R.J. Neufeld. 2004. Transport of gellan gum microbeads in soil columns of various grain size distributions. Can. J. Chem. Eng., 82: 994-1003.

81. Moslemy, P., S. Guiot and R.J. Neufeld. 2004. Activated sludge encapsulation in gellan gum microbeads for gasoline biodegradation. Bioprocess and Biosystems Engineering, 26: 197-204.

80. Raymond, M.-C., R.J. Neufeld and D. Poncelet. 2004. Encapsulation of brewers yeast in chitosan coated carrageenan microspheres by emulsification/thermal gelation. Artificial Cells, Blood Substitutes and Immobilization Biotechnology, 32: 275-292.

79. Belyaeva, Evgeniya, Dominique Della Valle, Ronald J. Neufeld and Denis Poncelet. 2004. New approach to the formulation of hydrogel beads by emulsification/thermal gelation using a static mixer. Chemical Engineering Science, 59: 2913-2920.

78. Gu, F., B. Amsden and R.J. Neufeld. 2004. Delivering vascular endothelial growth factor with alginate release system. J. Cont. Rel., 96: 463-472.

77. Decamps, C., S. Norton, D. Poncelet and R.J. Neufeld. 2004. Continuous process for the pilot plant scale immobilization of yeast in κ-carrageenan gel beads. AIChE J., 50: 1599-1605.

76. Moslemy, P, R.J. Neufeld, D. Millette and S.R. Guiot. 2003. Transport of gellan gum microbeads through sand: An experimental evaluation for encapsulated cell bioaugmentation. Journal of Environmental Management, 69: 249-259.

75. Yi, Y., S. Kermasha, L. L'Hocine and R.J. Neufeld. 2002. Encapsulation of Chlorophyllase in Hydrophobically Modified Hydrogel. J. Mol. Catalysis B: Enz. 809: 1-7.

74. Moslemy, P., R.J. Neufeld and S.R. Guiot. 2002. Biodegradation of gasoline by gellan gum-encapsulated bacterial cells. Biotechnol. Bioeng., 80: 175-184.

73. Moslemy, P., S.R. Guiot and R.J. Neufeld. 2002. Production of size-controlled gellan gum microbeads encapsulating gasoline-degrading bacteria. Enz. Microb. Technol., 30: 10-18.

72. DeGroot, A.R. and R.J. Neufeld. 2001. Encapsulation of urease in alginate beads and protection from a-chymotrypsin with chitosan membranes. Enz. Microb. Technol., 29: 321-327.

71. Boadi, D. and R.J. Neufeld. 2001. Encapsulation of tannase for the hydrolysis of tea tannins. Enz. Microb. Technol., 28: 590-595.

70. Ho, J. and R.J. Neufeld. 2001. Retention and release of low molecular weight DNA from alginate beads. invited paper. S.T.P. Pharma Sciences, 11(1): 109-115 (Thematic issue on gene and nucleic acid delivery).

Refereed Book Chapters And Reviews

18. Pinto Reis, C., R.J. Neufeld, F. Veiga and A.J. Ribeiro. Preparation of drug-loaded nanoparticles. Compilation of most influential and cited papers in nanomedicine, invited, updated and to be published with permission of Elsevier, Balogh, L.P., ed., Nanomedicine in Cancer: Nanomedicine most cited, Pan Stanford Publ., in press, 2016.

17. Haxho, F., R.J. Neufeld and M.R. Szewczuk. 2016. Neuraminidase-1: A novel therapeutic target in multistage tumorigenesis. Oncotarget, 7: 40860-40881.

16. Haxho, F., F. Alghamdi, R.J. Neufeld and M.R. Szewczuk. 2014. A novel insulin receptor-signaling platform. Invited review. Diabetes and Clinical Research, 1: 1-10.

15. Hrynyk, M. and R.J. Neufeld. 2014. Insulin and Wound Healing. Burns, 40: 1433-1446.

14. Steele, J.A.M., J-P. Hallé, D. Poncelet and R.J. Neufeld. 2014. Therapeutic cell encapsulation techniques and applications in diabetes. Advanced Drug Delivery Reviews, 67-68: 74-83. (invited review chapter; 5 year impact factor 15.413) (Featured on MDLinx.com, ranked by physician editors as #10 on pharma-news site).

13. Bowey, K., and R.J. Neufeld. 2010. Systemic and mucosal delivery of drugs within polymeric microparticles produced by spray drying. BioDrugs, 24: 359-377.

12. Pinto Reis, C., V. Veiga, A. Ribeiro and R.J. Neufeld. Design innovation for insulin delivery systems. In: The Bioartificial Pancreas and other biohybrid therapies. Hallé, J.-P., P. de Vos and L. Rosenberg (Eds.), Transworld Research Network, Kerala, IN, pp. 557-585 (2009).

11. Yi, Yunyu, R.J. Neufeld and D. Poncelet. Immobilization of Cells in Polysaccharide Gels. In: Polysaccharides: Structural Diversity and Functional Versatility, 2nd Edition, Dumitriu, S., (ed), Marcel Dekker Inc., NY. Invited review chapter, pp. 867-891 (2005).

10. Moslemy, P., S.R. Guiot and R.J. Neufeld. Encapsulation of bacteria for biodegradation of gasoline hydrocarbons. In: Methods in Biotechnology, 2nd Ed: Immobilization of Enzymes and Cells, Guisan J. M. (Ed.), Humana Press Inc., Totowa, NJ, USA, Invited chapter, pp. 415-426 (2006).

9. Neufeld, R.J. and D. Poncelet. Industrial scale encapsulation of cells using emulsification/dispersion technologies. In: Cell Immobilisation Biotechnology, Nedovic, V. and R. Willaert (eds), Focus on Biotechnology series, Vol. 8A, Kluwer Academic Publishers. Invited review chapter. pp. 311-326 (2004).